Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy

Table 2

Day 60 pregnancy and birth predictions on PCA transformed spectra data derived from metabolomic analysis of plasma recovered on Day 0 and Day 7 from recipients prior to transfer on Day 7 of embryos that had been cultured in vitro for 24 h in two laboratories (UNCEIA, France, and UTE-Bos, Spain).

Laboratory	Plasma analyzed	Pregnancy endpoint	𝑁	Positive	Negative	𝑘	PCA	Accuracy	Sensitivity	Specificity	AUC
France	Day 0	Day 60	25	15	10	3	−	59.6±4.4	58.7±6.9	61.0±5.7	0.657±0.033
		Birth	25	13	12	3	−	66.4±7.1	73.1±15.5	59.1±9.2	0.690±0.044
France	Day 7–Day 0	Day 60	25	15	10	3	−				<0.6
		Birth	25	13	12	3	−	72.0±3.8	69.7±10.2	75.0±8.8	0.789±0.032
Spain	Day 0	Day 60	23	13	10	1	−	69.1±5.2	72.8±9.1	62.0±6.3	0.671±0.067
		Birth	23	12	11	1	−				<0.55
Spain	Day 7–Day 0	Day 60	23	13	10	3	+	68.3±9.6	73.8±15.5	61.0±3.2	0.639±0.047
		Birth	23	12	11	3	+	67.8±3.0	69.2±5.6	66.3±4.4	0.662±0.021
France + Spain	Day 0	Day 60	48	28	20	1	+	74.2±1.1	78.2±3.1	70.7±1.1	0.766±0.014
		Birth	48	25	23	1	+	72.1±2.0	79.2±7.3	64.4±7.0	0.747±0.032
France + Spain	Day 7–Day 0	Day 60	48	28	20	3	+				<0.6
		Birth	48	25	23	3	+	69.8±6.0	58.4±3.4	82.2±9.5	0.657±0.033

𝑁: culture medium or plasma samples analyzed. Positive: samples that correspond to pregnancy/birth. Negative: samples that do not correspond to pregnancy/birth.
𝑘: adjustable model parameter of 𝑘-nearest neighbor classification algorithm.
PCA: principal component analysis (+: applied; −: did not improve the results when applied).
Day 7 plasma alone (not represented) yields <0.6 AUC.