Anti-Inflammatory Effects of the Nicotinergic Peptides SLURP-1 and SLURP-2 on Human Intestinal Epithelial Cells and Immunocytes
Figure 1
Anti-inflammatory effects of rSLURP-1 and -2 on IEC. The anti-inflammatory effects of 0.01 μg/mL of rSLURP-1 (S1) and -2 (S2) on secretion of IL-6, IL-8, and CXCL10 (ELISA) and expression of ICAM-1 (QIA) by CCL-241 and CCL-248 stimulated for 16 h in a humid, 5% CO2 incubator at a cell density of 1 × 106 cells/well with 100 U/mL of IL-1β (IL-6 assay), 25 μg/mL of the TLR9 ligand E. coli DNA (IL-8), 100 ng/mL of the TLR4 ligand LPS-EK (CXCL10), or 100 U/mL of INFγ (ICAM-1) were measured as described in Materials and Methods. Some cells were exposed to S1 or S2 in the presence of 1 μg/mL of anti-SLURP-1 or -2 monoclonal antibodies (Ab). Each experiment was performed in triplicate. Asterisk = , compared to untreated cells. Pound sign = , compared to an inflammatory stimulant given alone.
