Expression of Genes Encoding Enzymes Involved in the One Carbon Cycle in Rat Placenta is Determined by Maternal Micronutrients (Folic Acid, Vitamin B₁₂) and Omega-3 Fatty Acids

Figure 3

Rat placental mRNA levels of key enzyme genes in the one carbon cycle. (a) MTHFR levels; (b) MTR levels; (c) MAT2a levels; (d) CBS levels; (e) PEMT levels. Values are expressed as Mean ± SD (𝑛=8 for all). Significance: *𝑃<0.01 when compared to control (normal folate, normal B₁₂); ^#𝑃<0.01 when compared to NFBD (normal folate, B₁₂ deficient); ^$𝑃<0.01 when compared to EFB (excess folate, normal B₁₂), ^@𝑃<0.01 when compared to EFBD (excess folate, B₁₂ deficient); methylene tetrahydrofolate reductase (MTHFR), methionine tetrahydrofolate reductase (MTR), methionine adenosyl transferase (MAT), cystathionine b-synthase (CBS), and Phosphatidylethanolamine-N-methyltransferase (PEMT). NFB: normal folic acid, normal vitamin B₁₂; NFBD: normal folic acid, vitamin B₁₂ deficient; EFB: excess folic acid, normal vitamin B₁₂; EFBD: excess folic acid, vitamin B₁₂ deficient; NFBDO: normal folic acid, vitamin B₁₂ deficient, omega-3 supplemented EFBDO: excess folic acid, vitamin B₁₂ deficient, omega-3 supplemented.

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