Phenotypic Characterization of Mycoplasma synoviae Induced Changes in the Metabolic and Sensitivity Profile of In Vitro Infected Chicken Chondrocytes

Table 1

Metabolic and sensitivity profiles of CCH and CCH exposed to M. synoviae WVU 1853 membrane fraction.

C/N sources		CCH25+MSm^2	Cytotoxic/anti-cancer agents		CCH25+MSm^2
2,3-butanediol	−	+	12,4′-demethylepipodophyllotoxin	−	+
Acetic acid	−	+	Acivicin	−	+
a-D-glucose	+	↑	Aclarubicin	−	+
a-D-glucose-1-phosphate	−	+	Aklavine hydrochloride	−	+
Adonitol	−	+	Beta-peltatin	−	+
a-Hydroxy-butyric acid	−	+	Carmofur	+	−
a-Keto-glutaric acid	+	↑	Celastrol	−	+
a-Methyl-D-glucoside	+	=	Cepharanthine	−	+
a-Methyl-D-mannoside	+	=	Chloroquine diphosphate	−	+
b-Gentiobiose	+	−	Colchicine	−	+
b-Methyl-D-galactoside	−	+	Cytosine-beta-d-arabinofuranoside	−	+
b-Methyl-D-glucoside	−	+	Dactinomycin	−	+
b-Methyl-D-xylopyranoside	−	+	Daunorubicin hydrochloride	−	+
Chondroitin-6-sulfate	+	↑	Doxorubicin hydrochloride	−	+
D,L-b-Hydroxy-butyric acid	−	+	Elaidylphosphocholine	−	+
D,L-Lactic acid	+	↑	Emetine	−	+
D-Arabinose	−	+	Gossypol	−	+
D-Cellobiose	+	=	Indole-3-carbinol	−	+
Dextrin	−	+	Mercaptopurine	+	↑
D-Fructose	−	+	Mitoxantrone hydrochloride	−	+
D-Fructose-6-phosphate	+	=	Nocodazole	−	+
D-Fucose	−	+	Perillyl alcohol	+	↑
D-Glucosaminic acid	−	+	Piceatannol	−	+
D-Glucose-6-phosphate	+	↑	Picropodophyllotoxin	−	+
D-Lactitol	−	+	Podofilox	−	+
D-Maltose	+	↑	Quercetin dihydrate	−	+
D-Mannitol	−	+	Quinacrine hydrochloride	−	+
D-Mannose	−	+	Rapamycin	−	+
D-Melezitose	−	+	Rifaximin	+	−
D-Raffinose	+	=	Rotenone	−	+
D-Tagatose	−	+	Vinblastine sulfate	−	+
D-Trehalose	+	=
g-Amino-N-butyric acid	−	+	Ions
g-Hydroxy-butyric acid	−	+	Ammonium chloride	+	−
Glycogen	+	↑	Ferric chloride	+	↑
Hexanoic acid	−	+	Iodine	+	−
Lactulose	−	+	Lithium chloride	+	=
L-Glucose	−	+	Magnesium chloride	+	+
L-Glutamine	−	+	NaCl	+	−
L-Rhamnose	−	+	Potassium chloride	+	−
Malitol	+	=	Potassium chromate	+	−
Maltotriose	+	↑	Sodium molybdate	+	−
Melibionic acid	−	+	Sodium nitrate	+	−
Meso-tartaric acid	−	+	Sodium nitrite	+	−
Mono-methylsuccinate	−	+	Sodium pyrophosphate	+	−
Myo-inositol	−	+	Sodium sulfate	+	−
N-Acetyl-b-D-mannosamine	−	+	Zinc chloride	+	−
N-Acetyl-D-glucosamine	−	+
N-Acetylneuraminic acid	−	+
Pectin	−	+
Stachylose	−	+
Succinamic acid	−	+
Sucrose	+	=
Tricarballylic acid	−	+
18 dipeptides^3	+	−
10 dipeptides^4	+	=
3 dipeptides^5	+	↑
11 dipeptides^6	−	+

¹Respiration levels for CCH incubated for 25 h (CCH₂₅) were determined by comparing respiratory rates of CCH in each well of a plate to the corresponding A1 well that contained no supplements (+ respiration was higher than in A1).
²Respiration levels for CCH exposed to M. synoviae WVU 1853 membrane fraction (1 mg/mL, 15 µL/well for 5 h before the measurement started) were determined by comparing respiratory rates measured during 24 h to those of CCH₂₅.
³Dipeptides: Asn-Glu, Asp-Glu, Glu-Ala, Glu-Asp, Gln-Glu, Gly-Ala, Gly-Arg, Met-Trp, Phe-Ile, Phe-Val, Pro-Glu, Pro-Gln, Ser-Tyr, Thr-Pro, Thr-Ser, Trp-Val, Val-Phe, and Val-Ser.
⁴Dipeptides: Asp-Trp, Asp-Val, Glu-Ser, Glu-Tyr, Glu-Val, Gln-Gly, Tyr-Gln, Tyr-Glu, Tyr-Tyr, and Val-Lys.
⁵Dipeptides: Asp-Phe, Gln-Gln, and Gly-Asp.
⁶Dipeptides: Ala-Val, Arg-Trp, Arg-Tyr, Asp-Gly, Gly-Ser, Phe-Asp, Tyr-Val, Val-Ala, Val-Asn, Val-Gln, and Val-Gly.
Legend:
+: Respiration above cut-off value set to 500 (area under corresponding graph: for CCH compared to A1, for CCH+MSm compared to CCH).
−: Respiration lower than 500.
↑: Respiration of CCH+MSm higher than respiration of CCH in the presence of the same compound.
↓: Respiration of CCH+MSm lower than respiration of CCH in the presence of the same compound.
=: Respiration present in both CCH and CCH+MSm, difference not significant.