Mechanisms of Ascorbyl Radical Formation in Human Platelet-Rich Plasma
Effect of superoxide and the nitric oxide scavenger on formation in human PRP. ESR spectra (a) obtained from the reaction of (A) PRP (approximately cells/mL) and (B) superoxide scavenger (120 U/mL SOD and 1000 U/mL CAT) and the (C) nitric oxide scavenger (1 g/mL of hemoglobin) in the presence of 100 mM DMPO for 3 min. The ESR spectra are labeled to show their components: DMPO- adduct (*). The ESR signal intensity rates in the bar chart (b) are shown as the means ± SEM (). , compared with the control. The instrument parameters were identical to those shown in Figure 1(a).