BioMed Research International / 2014 / Article / Fig 7

Research Article

Mechanisms of Ascorbyl Radical Formation in Human Platelet-Rich Plasma

Figure 7

Effect of the fat-soluble (a) and water-soluble (b) enzyme inhibitor on hydroxyl free radical formation in the Fenton reaction. (a) The effect of the Fenton reaction solution (500  M hydrogen peroxide + 50  M FeSO4, positive control), 0.6% DMSO, 10  M DPI, 10  M AA861, 10  M L-NAME, 10  M allopurinol, 10  M clotrimazole, and 10  M indomethacin in the presence of 150 mM DMPO for 3 min on hydroxyl radical formation. (b) The effect of the Fenton reaction solution (positive control), 10  M quinacrine, and 100  M antimycin in the presence of 150 mM DMPO for 3 min on hydroxyl radical formation. The signal intensity rates and data shown in the bar chart are expressed as the means ± SEM ( ). compared with the solvent control. The instrument parameters were identical to those shown in Figure 1(a).
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(a)
614506.fig.007b
(b)