Mechanisms of Ascorbyl Radical Formation in Human Platelet-Rich Plasma
Effect of the fat-soluble (a) and water-soluble (b) enzyme inhibitor on hydroxyl free radical formation in the Fenton reaction. (a) The effect of the Fenton reaction solution (500 M hydrogen peroxide + 50 M FeSO4, positive control), 0.6% DMSO, 10 M DPI, 10 M AA861, 10 M L-NAME, 10 M allopurinol, 10 M clotrimazole, and 10 M indomethacin in the presence of 150 mM DMPO for 3 min on hydroxyl radical formation. (b) The effect of the Fenton reaction solution (positive control), 10 M quinacrine, and 100 M antimycin in the presence of 150 mM DMPO for 3 min on hydroxyl radical formation. The signal intensity rates and data shown in the bar chart are expressed as the means ± SEM (). compared with the solvent control. The instrument parameters were identical to those shown in Figure 1(a).