Estrogen-Related Receptor Alpha Confers Methotrexate Resistance via Attenuation of Reactive Oxygen Species Production and P53 Mediated Apoptosis in Osteosarcoma Cells
Figure 1
Enhanced ERRα increases resistance to MTX-induced cell death. (a) The expression of the ERRα gene was evaluated from nonmetastasis () and metastasis () osteosarcoma in the GEO dataset GSE21257. (b) Western blotting of ERRα gene in U2OS parental and U2OS ERRα overexpression stable cells (U2OS-ERRα). (c) U2OS parental and U2OS-ERRα (numbers 1 and 2) were treated with MTX (0-1 μM) for 48 hours and then survival rate was analyzed by counting cell numbers. (d) U2OS parental and U2OS-ERRα cells (numbers 1 and 2) were treated with 0.25 μM MTX and were collected at 24 hours, 48 hours, and 72 hours. The survival rate was analyzed by counting cell numbers. (e) U2OS parental and U2OS-ERRα cells (numbers 1 and 2) were treated with MTX (0-1 μM) for 72 hours; dead cells were assessed by the trypan blue exclusion assay. (f) U2OS parental and U2OS-ERRα cells (numbers 1 and 2) were treated with MTX (0-1 μM) for 48 hours and then cleaved caspase 7 and cleaved PARP were detected by Western blotting. Data are expressed as mean ± SD () and analyzed using Student’s -test. Asterisks indicate significant differences: , .
