Estrogen-Related Receptor Alpha Confers Methotrexate Resistance via Attenuation of Reactive Oxygen Species Production and P53 Mediated Apoptosis in Osteosarcoma Cells

Figure 3

ERRα confers MTX resistance by modulating ROS production in osteosarcoma cells. ((a)-(b)) U2OS parental and U2OS-ERRα cells (numbers 1 and 2) were treated with 1 μM MTX for 24 hours and then ROS production was analyzed by DCFH-DA staining using microreader and fluorescent microscope. (c) U2OS parental and U2OS-ERRα cells (numbers 1 and 2) were treated with different concentrations of H₂O₂ (0–100 mM) for 24 hours; cell numbers were counted. Data are expressed as mean ± SD (𝑛=3) and analyzed using Student’s 𝑡-test. Asterisks indicate significant differences: ^∗𝑃<0.05, ^∗∗𝑃<0.01.

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