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BioMed Research International
Volume 2014, Article ID 618096, 6 pages
http://dx.doi.org/10.1155/2014/618096
Research Article

Biodistribution and SPECT Imaging Study of 99mTc Labeling NGR Peptide in Nude Mice Bearing Human HepG2 Hepatoma

Department of Nuclear Medicine, Xijing Hospital, Fourth Military Medical University, No. 127 West Changle Road, Xi’an, Shaanxi Province 710032, China

Received 16 December 2013; Revised 30 January 2014; Accepted 3 February 2014; Published 19 May 2014

Academic Editor: Yongdoo Choi

Copyright © 2014 Wenhui Ma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A peptide containing Asn-Gly-Arg(NGR) sequence was synthesized and directly labeled with . Its radiochemical characteristics, biodistribution, and SPECT imaging were evaluated in nude mice bearing human HepG2 hepatoma. Nude mice bearing HepG2 were randomly divided into 5 groups with 5 mice in each group and injected with ~7.4 MBq . The SPECT images were acquired in 1, 4, 8, and 12 h postinjection via caudal vein. The metabolism of tracers was determined in major organs at different time points, which demonstrated rapid, significant tumor uptake and slow tumor washout. The control group mice were blocked by coinjecting unlabelled NGR (20 mg/kg). Tumor uptake was ( ) ID/g at 1 h, with the highest uptake of ( ) ID/g at 8 h. In comparison, the uptake of the blocked control group was ( ) ID/g at 1 h after injection. The SPECT static images and the tumor/muscle (T/NT) value were obtained. The highest T/NT value was at 8 h. The xenografted tumor became visible at 1 h and the clearest image of the tumor was observed at 8 h. In conclusion, can be efficiently prepared and it exhibited good properties for the potential SPECT imaging agent of tumor.