Rhubarb Tannins Extract Inhibits the Expression of Aquaporins 2 and 3 in Magnesium Sulphate-Induced Diarrhoea Model
Figure 2
Rhubarb tannins extract (RTE) has the antidiarrhoeal activity in magnesium sulphate- (MgSO4-) induced diarrhoea mice. Mice were orally administrated with RTE (125, 250, and 500 mg/kg, resp.) or water daily for 3 days before MgSO4-induced diarrhoea. During the experiment, fecal water content and evacuation index (EI) of mice defecation were evaluated. (a) Doses of 125~500 mg/kg RTE significantly decreased the fecal water content dose-dependently compared with vehicle-treated diarrhoea mice. (b) Doses of 125~500 mg/kg RTE significantly decreased the EI of defecation in a dose-dependent manner compared with vehicle-treated diarrhoea mice. (c) Macroscopic evidence of watery stool in colon was markedly observed in vehicle-treated diarrhoea mice, while dose of 125~500 mg/kg RTE significantly alleviated the watery stool of diarrhoea mice. (d) Histological structure of colon in mice induced by MgSO4 appeared edematous while RTE treatment seemed to reduce edema. Data are represented as the mean ± SD. ### versus normal control group. , , and versus vehicle group, respectively. in each group and each assay was repeated 3 times.
