BioMed Research International / 2014 / Article / Fig 7

Research Article

Rhubarb Tannins Extract Inhibits the Expression of Aquaporins 2 and 3 in Magnesium Sulphate-Induced Diarrhoea Model

Figure 7

Rhubarb tannins extract (RTE) downregulates the expression of magnesium sulphate- (MgSO4-) activated PKA and p-CREB in HT-29 cells without effect on cell viability. Cells were placed in 100-mm dishes for 24 h in the presence of MgSO4 with or without RTE (control, MgSO4, and RTE- (20, 40, and 80 μg/mL) treatment groups, resp.). HT-29 cells were collected to detect the expression levels of PKA (a) and p-CREB (b) protein by western blot analysis. (c) No effect of RTE (20, 40, 80 μg/mL, resp.) on the cell viability by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Cell viability of the control was taken as 100%. Data are represented as the mean ± SD. ### versus normal control group. , , and versus vehicle group, respectively. in each group and each assay was repeated 3 times.
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