Nek2 Is a Novel Regulator of B Cell Development and Immunological Response

Figure 1

Generation of transgenic mice with B cell lineage specific overexpression of Nek2. (a) Illustration of construct used to generate transgenic mice. (b) Nek2 Tg mice were bred with CD19-Cre mice and genotyped with F1 + R1, F2 + R2, and Cre primers. (c) CD19 positive cells were sorted from transgene positive mice and its littermate controls (). Expression of Nek2 mRNA was detected by RT-qPCR. (d) Expression of Nek2 was detected by western blots in CD19 positive sorted cells.

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