Research Article
Nek2 Is a Novel Regulator of B Cell Development and Immunological Response
Figure 2
Analysis of B cell development in Nek2 Tg mice. (a) Hardy fractions from bone marrow (fraction (A) to (F)) were gated as follows: (A) B220+CD43+BP-1−CD24−; (B) B220+CD43+BP-1−CD24+; (C) B220+CD43+BP-1+CD24+; (D) B220+CD43−IgM−IgD−; (E) B220+CD43−IgM−IgD+; and (F) B220+CD43−IgM+IgD+. Fractions (A) and (B)–(D) may also be gated as CD19− and CD19+ populations among B220+IgM− cells, respectively (b) IgM+ splenocytes were separated into the following subsets: (T1), CD93+CD23−; (T2), CD93+CD23+Ig; marginal zone (MZ), CD93−CD23−IgCD2; and follicular (Fo), CD93−CD23+. (c) Peritoneal lymphocytes were separated into the following subsets: (B-2), CD19+B22; (B-1), CD19+B22; (B-1a), CD5+CD43+; and (B-1b), CD5−CD43−.
(a) Bone marrow |
(b) Spleen |
(c) Peritoneal cavity |