BioMed Research International / 2014 / Article / Tab 5 / Research Article
Differential Expression of Cholecystokinin A Receptor in Gallbladder Cancer in the Young and Elderly Suggests Two Subsets of the Same Disease? Table 5 CCKAR expression profile in human malignancies: literature review. CCKAR: cholecystokinin type A receptor.
First author Tumour types Number of samples Methodology Findings Okada [19 ] Gastric 14 RT-PCR Suggest a greater role for CCK and CCKAR than for gastrin and CCK-B receptor in gastric cancers Clerc [15 ] Oesophageal, gastric, and colon cancer 8: oesophageal 12: colon 8: gastric RT-PCR The expression of CCKAR may be an important indicator of the influence of CCK on the origin and growth of these cancers Reubi [20 ] Various human malignancies 32: gastroenteropancreatic tumour 24: medullary thyroid carcinoma 16: neuroblastoma 27: meningioma 65: breast carcinoma Receptor autoradiography CCKAR rarely expressed in tumors except gastroenteropancreatic tumors (38%), meningiomas (30%), and some neuroblastomas (19%) Weinberg [21 ] Pancreatic cancer 22 RT-PCR Overexpression of CCKAR mRNA in pancreatic cancer Moonka [16 ] Pancreatic cancer 30 RT-PCR Increased expression of CCKAR mRNA may stimulate pancreatic cancer Schulz [22 ] Various human tumours 5: colorectal 5: pancreatic adenocarcinoma 5: breast 10: ovarian 4: prostate 6: thyroid 15: carcinoid 8: pancreatic insulinoma 4: pituitary adenoma 4: pheochromocytoma 4: glioblastoma 4: meningioma IHC CCKAR overexpression in a subset of human neuroendocrine tumours may provide a molecular basis for efficient targeting of these tumors with radiolabeled CCK analogs Rai [23 ] Gallbladder cancer 94 IHC Significant increase in expression of CCKAR in gallbladder cancer as compared to gallstone disease Current study Gallbladder cancer 31 resected samples RT-PCR, real-time PCR, and ELISA Overexpression of CCKAR mRNA and protein in GBC tissues as compared with normal gallbladder suggests its therapeutic potential
RT-PCR: reverse transcriptase-polymerase chain reaction and IHC: immunohistochemistry.