OpenMebius: An Open Source Software for Isotopically Nonstationary 13C-Based Metabolic Flux Analysis
Figure 2
Procedure for metabolic flux estimation. Step 1: initial estimates of metabolic fluxes are given at random following the constraints of the mass balance, biomass synthetic rate, and substrate consumption and production rate. Step 2: metabolic fluxes are determined by minimizing the difference between simulated and measured isotopic labeling enrichment using the Levenberg-Marquardt method.
