Research Article
Biological Safety of Fish (Tilapia) Collagen
Table 1
Results of colony-formation inhibition assay.
(a) |
| Test area | Colonies/well | Mean ± SD | Colony-formation ratio (%) |
| Control (MEM10 medium) | 38, 43, 42, 40 | | 100 | Negative control material | | | | Plastic sheet, toluene resisting | 36, 40, 41, 41 | | 96.8 | Positive control material B | | | | Polyurethane film containing 0.25% ZDBC | 0, 0, 0, 0 | | 0.0 | Test article | | | | Fish collagen | 38, 40, 36, 39 | | 93.9 |
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(b) |
| Test solution | Concentration (μg/mL) | Colonies/well | Mean ± SD | Colony-formation ratio (%) | IC50 (μg/mL) |
| Control (DMSO) | 0 | 41, 37, 41, 44 | | 100 | — |
| Positive control article ZDBC | 1 | 37, 43, 37, 41 | | 96.8 | 1.60 | 2 | 5, 11, 6, 6 | | 17.2 | 3 | 0, 0, 0, 0 | | 0.0 |
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ZDBC: Zinc dibutyldithiocarbamate. DMSO: Dimethyl sulfoxide.
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