Biological Safety of Fish (Tilapia) Collagen

Table 1

Results of colony-formation inhibition assay.

(a)

Test area	Colonies/well	Mean ± SD	Colony-formation ratio (%)
Control (MEM10 medium)	38, 43, 42, 40	40.8±2.2	100
Negative control material
Plastic sheet, toluene resisting	36, 40, 41, 41	39.5±2.4	96.8
Positive control material B
Polyurethane film containing 0.25% ZDBC	0, 0, 0, 0	0.0±0.0	0.0
Test article
Fish collagen	38, 40, 36, 39	38.3±1.7	93.9

(b)

Test solution	Concentration (μg/mL)	Colonies/well	Mean ± SD	Colony-formation ratio (%)	IC50 (μg/mL)
Control (DMSO)	0	41, 37, 41, 44	40.8±2.9	100	—
Positive control article; ZDBC	1	37, 43, 37, 41	39.5±3.0	96.8	1.60
	2	5, 11, 6, 6	7.0±2.7	17.2
	3	0, 0, 0, 0	0.0±0.0	0.0

ZDBC: Zinc dibutyldithiocarbamate.
DMSO: Dimethyl sulfoxide.