Use of Synthetic Single-Stranded Oligonucleotides as Artificial Test Soiling for Validation of Surgical Instrument Cleaning Processes

Figure 1

Overview of the quantification of the residual ssDNA_ODN amount on test objects after cleaning in a medical cleaning machine. Stainless steel test objects are coated with a defined amount of ssDNA_ODN and cleaned in a medical cleaning machine. Using 5 M urea solution at 93°C, residual ssDNA_ODN is eluted from the surface of test objects. Eluates are purified and concentrated using Amicon Ultra 30K centrifugal filters. The ssDNA_ODN amount is quantified using quantitative real-time PCR (qPCR).