Detection of Newcastle Disease Virus Minor Genetic Variants by Modified Single-Stranded Conformational Polymorphism Analysis

Figure 2

Multitemperature single-stranded conformational polymorphism (MSSCP) electrophoresis of 123 nt fusion protein fragments of two vaccine Newcastle disease virus strains obtained from RT-PCR (lanes 2–5 and 8–11) and PCR (lanes 6 and 7). Types of templates (nR, aR, and nD) were described in Section 2. Lanes: 1. Double-stranded pUC19 DNA/MspI Marker, 23 (Thermo Scientific, USA), 2. Oral swab nR La Sota, 3. Oral swab nR Roakin, 4. Cloacal swab nR La Sota, 5. Cloacal swab nR Roakin, 6. Plasmid nD La Sota, 7. Plasmid nD Roakin, 8. Allantoic fluid nR La Sota, 9. Allantoic fluid nR Roakin, 10. Transcribed RNA aR La Sota, 11. Transcribed RNA aR Roakin, 12. RT-PCR negative control, 13. PCR negative control. MSSCP was performed as described in Table 2 without the first two temperatures steps.