Apoptotic and Inhibitory Effects on Cell Proliferation of Hepatocellular Carcinoma HepG2 Cells by Methanol Leaf Extract of Costus speciosus
Figure 2
FACS analyses of Annexin V and 7-AAD staining. HepG2 cells were treated with methanolic extract of Costus speciosus leaves or sorafenib (100 μg/mL) for 24 h. Lower right quadrant (D4) Annexin V positive/7-AAD negative cells denote early apoptosis cells and upper right quadrant (D2) Annexin V positive/7-AAD positive cells denote necrosis or late apoptotic cells. Images are the representative of three independent experiments.
