Apoptotic and Inhibitory Effects on Cell Proliferation of Hepatocellular Carcinoma HepG2 Cells by Methanol Leaf Extract of Costus speciosus
Figure 4
Effect of methanolic extract of Costus speciosus leaves on mitochondrial membrane potential (). (a) Mitochondrial membrane potential () was measured by JC-1 fluorescence [fluorescence of JC-1 monomers (em 535 nm)/aggregates (em 590 nm)] in HepG2 cells treated with 100 μg/mL of methanol extract or sorafenib for 24 h. Methanol extract or sorafenib showed significant decrease in in HepG2 cells. Data are expressed as mean ± SD (). Columns not sharing the same superscript letter differ significantly (%). (b) Untreated control cells showed that cells have higher mitochondrial as they are abundant in J-aggregates which is indicated by intense red fluorescence; methanol extract or sorafenib treated cells showed lower mitochondrial which is represented by monomeric JC-1 with green fluorescence.
