rhPDGF-BB Promotes Proliferation and Osteogenic Differentiation of Bone Marrow Stromal Cells from Streptozotocin-Induced Diabetic Rats through ERK Pathway

<table>rhPDGF-BB-induced osteogenic differentiation in diabetic BMSCs was inhibited by suppression of ERK pathway. Cells were pre-treated with or without 20 <i><i>μ</i></i>M PD98059 for 1 h and treated with 10 ng/mL rhPDGF-BB. The unmanipulated cells were used as controls. (a) Real-time PCR analysis of ALP, Runx2, and OCN mRNA expression on day 7. (b) Western blot analysis for the relative Runx2 protein levels on day 7. (c) ALP staining on day 14 (16x). (d) Quantitative results of ALP assay on day 14. (e) Alizarin red staining for mineralized nodules on day 21 (50x). *<svg height="11.375" id="M15" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 58.712502 11.375" width="58.712502" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>, versus control, and <sup>#</sup><svg height="11.375" id="M16" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 58.712502 11.375" width="58.712502" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>, versus 10 ng/mL.</table>

BioMed Research International

fig5

Figure 5

Figure 5: rhPDGF-BB Promotes Proliferation and Osteogenic Differentiation of Bone Marrow Stromal Cells from Streptozotocin-Induced Diabetic Rats through ERK Pathway 

Figure 5 | rhPDGF-BB Promotes Proliferation and Osteogenic Differentiation of Bone Marrow Stromal Cells from Streptozotocin-Induced Diabetic Rats through ERK Pathway 