Sequence Variation in the E2-Binding Domain of HPV16 and Biological Function Evaluation in Tunisian Cervical Cancers

Table 1

Polymerase chain reaction and reverse transcription primers, product length, and programs.

	Designation	Product length (bp)	PCR program
Primers for PCR assay
𝛽-Globin sense	5′-CAACTTCATCCACGTTCACC-3′	268	95°C 1′, 55°C 1′, 72°C 1′; X 40
𝛽-Globin antisense	5′-GAAGAGCCAAGGACAGGTAC-3′
HPV16 L1 sense	5′-GCAAGCAACAGTTACTGCGACGT-3′	301	94°C 1′, 58°C 1′, 72°C 1′; X 40
HPV16 L1 antisense	5′-GCAACAAGACATACATCGACCGG-3′
HPV16 E2 sense	5′-ATGAAAATGATAGTACAGAC-3′	1026	95°C 1′, 50°C 2′, 72°C 1′ 30 s; X 35
HPV16 E2 antisense	5′-CCAGTAGACACTGTAATAG-3′
Primers for RT-PCR assay
𝛽-Actin sense	5′-AGCCATGTACGTTGCTATCC-3′	500	94°C 30 s, 50°C 30 s, 72°C 1′; X 30
𝛽-Actin antisense	5′-TTGGCGTACAGGTCTTTGC-3′
HPV16 E6 sense	5′-TTACCACAGTTATGCACAGA-3′	300	94°C 30 s, 50°C 30 s, 72°C 1 min; X 30
HPV16 E6 antisense	5′-ACAGTGGCTTTTGACAGTTA-3′
HPV16 E7 sense	5′-AGAAACCCAGCTGTAATCAT-3′	300	94°C 30 s, 50°C 30 s, 72°C 1 min; X 30
HPV16 E7 antisense	5′-TTATGGTTTCTGAGAACAGA-3′