Detection of Genetic Variations in Coagulopathy-Related Genes Using Ramified Rolling Circle Amplification
Figure 3
Representative plots of RAM assays performed on control DNA samples. The data points represent RAM signals (Rts) from C-probes ligated to genome equivalents. Green data points represent Wt samples (wild type response times, “Wt Rt”); red data points represent homozygous Mt samples samples (mutant response times, “Mt Rt”); purple data points represent heterozygotes. “NR” on the axis scales represents “No Response”. Each homozygous sample response time is plotted on its labeled axis and at the no-response level on the alternate axis; for example, wild type (Wt) data points are plotted at on the “Wt” axis and at “NR” on the “Mt” axis. “Rt”, response time. MTHFR 677 C-T “Mt” homozygous samples done in a separate assay have a higher mean response time than the same C-probes applied to the heterozygous samples. Separate measurements for each allele from heterozygous samples are plotted within the panels as example pairings of allele measurements.
