Effects of Growth Phase and Temperature on Activity within a Listeria monocytogenes Population: Evidence for RsbV-Independent Activation of at Refrigeration Temperatures

Table 1

Plasmids and strains used in this study.

Plasmid	Abbreviated name	Source or reference	Collection number
Champion pET101 directional TOPO expression  vector containing topoisomerase for efficient and simple cloning	pET101D	Invitrogen	N/A
Champion pET101 directional TOPO expression  vector with wild type rsbW with N-terminal His6-tag	pEC02	This study	N/A
Champion pET101 directional TOPO expression  vector with wild type sigB with N-terminal His6-tag	pEC03	This study	N/A
Strains
One shot TOP10 chemically competent E. coli  F^−  mcrA Δ(mmr-hsdRMA-mcrBC) Φ80lacZΔM15  ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK  rpsL (Str^R) endA1 nupG	E. coli TOP10	Invitrogen	N/A
BL21 Star (DE3) one shot chemically Competent E.  coli  F^−  impT hsdSB (rB^−mB^−) gal  dcm  rne131 (DE3)	E. coli BL21 (DE3)	Invitrogen	N/A
BL21 Star (DE3) E. coli/pEC02	E. coli/rsbW-his6	This study	COB275
BL21 Star (DE3) E. coli/pEC03	E. coli/sigB-his6	This study	COB276
L. monocytogenes EGD-e	wt	K. Boor	COB261
L. monocytogenes EGD-e Δ sigB	ΔsigB	K. Boor	COB262
L. monocytogenes EGD-e Δ rsbV	ΔrsbV	Utratna et al. 2012 [24]	COB411
L. monocytogenes EGD-e::pKSV7-P𝑙𝑚𝑜2230::egfp	wt-egfp	Utratna et al. 2012 [24]	COB491
L. monocytogenes EGD-e ΔsigB::pKSV7-P𝑙𝑚𝑜2230::egfp	ΔsigB-egfp	Utratna et al. 2012 [24]	COB495
L. monocytogenes EGD-e ΔrsbV::pKSV7-P𝑙𝑚𝑜2230::egfp	ΔrsbV-egfp	Utratna et al. 2012 [24]	COB501