Effects of Growth Phase and Temperature on Activity within a Listeria monocytogenes Population: Evidence for RsbV-Independent Activation of at Refrigeration Temperatures
Table 1
Plasmids and strains used in this study.
Plasmid
Abbreviated name
Source or reference
Collection number
Champion pET101 directional TOPO expression vector containing topoisomerase for efficient and simple cloning
pET101D
Invitrogen
N/A
Champion pET101 directional TOPO expression vector with wild type rsbW with N-terminal His6-tag
pEC02
This study
N/A
Champion pET101 directional TOPO expression vector with wild type sigB with N-terminal His6-tag
pEC03
This study
N/A
Strains
One shot TOP10 chemically competent E. coli F− mcrA (mmr-hsdRMA-mcrBC) Φ80lacZΔM15 lacX74 recA1 araD139 (ara-leu)7697 galU galK rpsL (StrR) endA1 nupG
E. coli TOP10
Invitrogen
N/A
BL21 Star (DE3) one shot chemically Competent E. coli F− impT hsdSB (rB−mB−) gal dcm rne131 (DE3)