Early Appearance of Nonvisual and Circadian Markers in the Developing Inner Retinal Cells of Chicken
Figure 3
Immunocytochemistry for Prox-1, Opn4x, and α-Tubulin proteins staining and merge in HC cultures at E15. Individual cells from primary cultures of HCs were obtained by the 2.5% phase of a bovine albumin serum (BSA) gradientpurification, maintained for 48–72 h, immunolabeled for Opn4x-like protein ((b) red), α-Tubulin (DM1A) ((a) green), Prox-1 ((a) red; (b) green), and nuclei staining by DAPI (blue), and then visualized by confocal microscopy as described in Section 2.
