Upregulation of Transglutaminase and ε (γ-Glutamyl)-Lysine in the Fisher-Lewis Rat Model of Chronic Allograft Nephropathy

Figure 5

Representative fields of immunofluorescence staining (FITC, green) for transglutaminase type 2 protein ((a)–(d)) and ε(γ-glutamyl)-lysine ((g)–(j)) for the Lewis- (L-) Lewis (L) isograft and Fisher- (F-) Lewis (L) allograft in both glomerular and tubulointerstitial areas. Transglutaminase type 2 protein ((e)–(f)) and ε(γ-glutamyl)-lysine cross-link ((k)–(l)) were quantified by multiphase image analysis and expressed as the FITC/DAPI ratio for L-L isografts and F-L allografts at termination and for control F and L rats. Vertical bars indicate ± SEM.

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