Upregulation of Transglutaminase and ε
(γ-Glutamyl)-Lysine in the Fisher-Lewis Rat Model of
Chronic Allograft Nephropathy
Representative fields of immunofluorescence staining (FITC, green) for transglutaminase type 2 protein ((a)–(d)) and ε(γ-glutamyl)-lysine ((g)–(j)) for the Lewis- (L-) Lewis (L) isograft and Fisher- (F-) Lewis (L) allograft in both glomerular and tubulointerstitial areas. Transglutaminase type 2 protein ((e)–(f)) and ε(γ-glutamyl)-lysine cross-link ((k)–(l)) were quantified by multiphase image analysis and expressed as the FITC/DAPI ratio for L-L isografts and F-L allografts at termination and for control F and L rats. Vertical bars indicate ± SEM.