Phenotypic Switch Induced by Simulated Microgravity on MDA-MB-231 Breast Cancer Cells
Figure 4
Cell cycle analysis in MDA-MB-231. Cells distribution along the different phases of the cell cycle at 24 (a) and 72 hours (b), in on ground control cells, RPM adherent cells, and RPM cell clumps. (c) Immunoblot bar chart showing the expression of Cyclin D1 in MDA-MB-231 in on ground control cells, RPM adherent cells, and RPM cell clumps at 24 and 72 hours. Columns and bars represent densitometric quantification of optical density (OD) of specific protein signal normalized with the OD values of the GAPDH served as loading control. Each column represents the mean value ± SD of three independent experiments. ; ; versus on ground control; ; versus RPM adherent cells by ANOVA followed by Bonferroni post-test.
