Review Article
Application of PCR-ELISA in Molecular Diagnosis
Table 1
Comparisons between 3 different detection methods; conventional PCR with agarose gel electrophoresis, PCR-ELISA and qPCR.
| Comparison | Conventional PCR | PCR-ELISA | qPCR |
| Equipment required | Standard laboratory equipment | Standard laboratory equipment | Requires fluorescence detection instrument | Reagent costs | Low | Moderate | Costly | Detection limit | 1–10 ng/μL | 0.01 ng/μL | 0.25 pg/μL | Quantitative ability | Not quantitative | Semi-quantitative | Quantitative |
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