Regulation of Melanopsins and Per1 by α-MSH and Melatonin in Photosensitive Xenopus laevis Melanophores

Table 1

Primers and probes used for qPCR assays.

18S RNA	Forward: 5′-CGGCTACCACATCCAAGGAA-3′
	Backward: 5′-GCTGGAATTACCGCGGCT-3′
	Probe: 5′-5-TexRd-TGCTGGCACCAGACTTGCCCTC-BHQ_2-3′
Opn4x; NM_00108564.1	Forward: 5′-ATTATTGTCCTTGTCTGGATGTATTCA-3′
	Backward: 5′-AAGCCCTCTGGCACATAGGAA-3′
	Probe: 5′-6-FAM-AATGTGGAGCTTGGCACCATTACTTGGC-BHQ_1-3′
Opn4m; DQ_384639.1	Forward: 5′-AGGGCAGTGCTAATCCTTTCAGGT-3′
	Backward: 5′-AATCCCAGGTGCAGGATGTCAGAA-3′
Per1; NM_001085703.2	Forward: 5′-TGAAGGCCCTTAAAGAGCTAAAGA-3′
	Backward: 5′-TTGCCAGTGTGCCAGACTTG-3′
	Probe: 5′-/5Cy5/TCGGCTCCCATCAGAGAAGAG GCTAAAAG/3BHQ_2/3′