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BioMed Research International
Volume 2014 (2014), Article ID 654712, 8 pages
Research Article

In Vitro Antiviral Activity of Circular Triple Helix Forming Oligonucleotide RNA towards Feline Infectious Peritonitis Virus Replication

1Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
2Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
3Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

Received 28 October 2013; Revised 2 January 2014; Accepted 14 January 2014; Published 20 February 2014

Academic Editor: Abdurrahman Saydut

Copyright © 2014 Oi Kuan Choong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1. NanoITC analysis. NanoITC analysis measures the binding affinity of circular TFO to its target region. Dissociation constant (Kd) is equal to 1/association constant (Ka). Supplementary Figure 2. Morphological changes of CRFK cells following TFO RNAs treatment. FIPV-infected cells exposed to TFOs which did not show cytopathic effect (CPE) indicated the inhibitory effects of the circular TFO against FIPV replication. Arrows show the CPE. 10X magnification. Supplementary Figure 3. In vitro antiviral activity of circular TFO. In vitro antiviral activity of TFO1 (A) and TFO5 (B) towards influenza A virus H1N1 replication showed no significant reduction in viral RNA genome copy numbers between the TFO RNA transfected cells and untransfected cells (p ≥ 0.05). Data are averages of 3 independent tests (mean ± SE). NS: not significant.

  1. Supplementary Figures