A Simple and Efficient Method to Isolate LTR Sequences of Plant Retrotransposon

Table 3

The programme parameters of three rounds of PCR.

Phase	Step	Cycle conditions	Cycles
First PCR round	1	94°C for 5 min	1
	2	94°C for 50 s, 60°C for 1 min, and 72°C for 2 min	5
	3	94°C for 50 s, 45°C for 30 s, and 72°C for 2 min	1
	4	94°C for 50 s, 55°C for 30 s, and 72°C for 2 min	25
	5	72°C for 8 min	1
Second PCR round	1	94°C for 5 min	1
	2	94°C for 50 s, 55°C for 30 s, and 72°C for 2 min	30
	3	72°C for 10 min	1
Third PCR round	1	94°C for 5 min	1
	2	94°C for 1 min, 55°C for 30 s, and 72°C for 2 min	30
	3	72°C for 10 min	1