A Simple and Efficient Method to Isolate LTR Sequences of Plant Retrotransposon

Table 4

The sequences of primers used in this study.

Primers		Sequence
Nested primer	RNase H1	MGNACNAARCAYATHGA
	RNase H2	GCNGAYATNYTNACNAA
ACP primer	ACP 1	TGTAGCGTGAAGACGACAGAA IIIII VNVNNNGGAA
	ACP 2	TGTAGCGTGAAGACGACAGAA IIIII BNBNNNGGTT
	ACP 3	TGTAGCGTGAAGACGACAGAA IIIII HNVNNNCCAC
	ACP 4	TGTAGCGTGAAGACGACAGAA IIIII CAATGGCTACCAC
	ACP 5	TGTAGCGTGAAGACGACAGAA IIIII VVNVNNNCCAA
	ACP 6	TGTAGCGTGAAGACGACAGAA IIIII BDNBNNNCGGT
UP primer	UP	TGTAGCGTGAAGACGACAGAA
PPT primer		RRRRRRRRRRRRRRRR

Note: the bold letters represent the nontarget universal sequences. I represent deoxyinosine. The meaning of degenerate base: B (C/G/T), D (A/G/T), H (A/C/T), R (A/G), N (A/G/C/T), and V (A/C/G).