The Step Further to Understand the Role of Cytosolic Phospholipase A2 Alpha and Group X Secretory Phospholipase A2 in Allergic Inflammation: Pilot Study
Concentration of sPLA2X released by PBMC of asthmatics and healthy controls. 2 × 106 PBMC were incubated in medium containing RPMI1640 with 10% heat-inactivated FBS and antibiotics (37°C, 5% CO2). Supernatants were collected after 24 hours of incubation in 37°C. The secretion of sPLA2X was measured in indirect ELISA. Data are presented as mean ± SEM; * as comparison between studied groups.