The Step Further to Understand the Role of Cytosolic Phospholipase A2 Alpha and Group X Secretory Phospholipase A2 in Allergic Inflammation: Pilot Study
Relative cPLA2 protein expression in PBMC from healthy subjects and asthmatic patients in vitro stimulated with rDer p1, rFel d1, and LPS in dose-dependent manner. PBMC (2 × 106) were stimulated with indicated doses of LPS or rDer p1 or rFel d1 for 24 hours. Control represents cells treated with the vehicle. The immunoblot is representative of experiments in PBMC from at least six donors, each showing similar results. The line graph shows the densitometry results obtained from cells stimulated with rFel d1. Data are presented as the fold change compared with the vehicle-treated cells (control). Data represent the mean ± SE from at least six independent experiments. *shows comparison between studied groups. HY: healthy subjects. SA: severe asthmatics.