Feleucins: Novel Bombinin Precursor-Encoded Nonapeptide Amides from the Skin Secretion of Bombina variegata

Table 1

Predicted b- and y-ion series (singly and doubly charged) arising from MS/MS fragmentation of (a) the novel bombinin, (b) feleucin-BV1, and (c) feleucin-BV2. Ions observed in actual MS/MS spectra are indicated in bold typeface.

(a)

Number 1	b(1+)	b(2+)	Seq.	y(1+)	y(2+)	Number 2
1	58.02875	29.51801	G			27
2	171.11282	86.06005	I	2560.50874	1280.75801	26
3	228.13429	114.57078	G	2447.42467	1224.21597	25
4	285.15576	143.08152	G	2390.40320	1195.70524	24
5	356.19288	178.60008	A	2333.38173	1167.19450	23
6	469.27695	235.14211	L	2262.34461	1131.67594	22
7	582.36102	291.68415	L	2149.26054	1075.13391	21
8	696.40395	348.70561	N	2036.17647	1018.59187	20
9	795.47237	398.23982	V	1922.13354	961.57041	19
10	852.49384	426.75056	G	1823.06512	912.03620	18
11	980.58881	490.79804	K	1766.04365	883.52546	17
12	1079.65723	540.33225	V	1637.94868	819.47798	16
13	1150.69435	575.85081	A	1538.88026	769.94377	15
14	1263.77842	632.39285	L	1467.84314	734.42521	14
15	1391.87339	696.44033	K	1354.75907	677.88317	13
16	1448.89486	724.95107	G	1226.66410	613.83569	12
17	1561.97893	781.49310	L	1169.64263	585.32495	11
18	1633.01605	817.01166	A	1056.55856	528.78292	10
19	1761.11102	881.05915	K	985.52144	493.26436	9
20	1818.13249	909.56988	G	857.42647	429.21687	8
21	1931.21656	966.11192	L	800.40500	400.70614	7
22	2002.25368	1001.63048	A	687.32093	344.16410	6
23	2131.29628	1066.15178	E	616.28381	308.64554	5
24	2268.35519	1134.68123	H	487.24121	244.12424	4
25	2415.42361	1208.21544	F	350.18230	175.59479	3
26	2486.46073	1243.73400	A	203.11388	102.06058	2
27			N-Amidated	132.07676	66.54202	1

(b)

Number 1	b(1+)	Seq.	y(1+)	Number 2
1	148.07570	F		9
2	261.15977	L	754.51859	8
3	318.18124	G	641.43452	7
4	431.26531	L	584.41305	6
5	544.34938	L	471.32898	5
6	601.37085	G	358.24491	4
7	658.39232	G	301.22344	3
8	771.47639	L	244.20197	2
9		L-Amidated	131.11790	1

(c)

Number 1	b(1+)	Seq.	y(1+)	Number 2
1	148.07570	F		9
2	261.15977	L	784.52915	8
3	318.18124	G	671.44508	7
4	431.26531	L	614.42361	6
5	544.34938	I	501.33954	5
6	601.37085	G	388.25547	4
7	688.40288	S	331.23400	3
8	801.48695	L	244.20197	2
9		L-Amidated	131.11790	1