BioMed Research International / 2014 / Article / Fig 3

Research Article

Collagen-Glycosaminoglycan Matrix Implantation Promotes Angiogenesis following Surgical Brain Trauma

Figure 3

Proliferating neurons defined with NeuN+/Ki67+ immunoreactivity in the lesion boundary zone (LBZ) of a surgical traumatic brain lesion with implantation of a collagen-glycosaminoglycan matrix on day 14. Demonstrative photomicrographs of double immunofluorescence staining of illustrative brain sections from L+CG rats on day 14 following surgical trauma. (a) Immunoreactivity of NeuN (green), a neuronal marker, and (b) Ki67 (red), which stains proliferating cells in the intra-matrix zone (IMZ) of the L+CG group. DAPI (diamidino-2-phenylindole) (blue), a classic nuclear counterstain for immunofluorescence, and merged image (c). Scale bars in (a–c) represent 75 μm. (d) Numbers of proliferative neurons (Ki67+/NeuN+) from brains of the L+CG group on D7, D14, D21 and D28 after surgery in the intra-matrix zone (IMZ). Data are the mean ± SD. , , and , L and L+CG groups versus the Sham group; +, ++ and +++, L versus L+CG.
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