Towards Scarless Wound Healing: A Comparison of Protein Expression between Human, Adult and Foetal Fibroblasts

Table 1

Variation in relative protein concentrations expressed by human adult (HAF) and foetal (HFF) dermal fibroblasts, as identified by mass spectrometry.

Spot number	Protein	Accession number	pI	pI	Mr	Mr	SIR	±
1	Alpha-enolase	P06733	7.01	7.10	47169	49000	1  : 0.86	0.12
2	Alpha-enolase	P06733	7.01	7.61	47169	49000	1 : 0.93	0.09
3	Alpha-enolase	P06733	7.01	8.00	47169	49000	1 : 0.84	0.11
4	3-ketoacyl-CoA thiolase, mitochondrial	P42765	8.32	9.00	41924	47000	1 : 0.11	0.09*
5	Fructose-bisphosphate aldolase A^●	P04075	8.30	7.34	39420	40000	1 : 0.68	0.07*
6	Cofilin-1^●	P23528	8.22	6.85	18502	20000	1 : 0.57	0.07*
7	Peroxiredoxin-1^●	Q06830	8.27	8.17	22110	23000	1 : 0.45	0.08*
8	Peptidyl-prolyl cis-trans isomerase B	P23284	9.60	9.50	22068	24000	1 : 0.83	0.10*
9	Lactotransferrin^●	P02788	8.50	8.67	78182	10000	1 : 0.71	0.05*
10	Glucosidase 2 subunit beta	P14314	4.33	4.34	59425	65000	1 : 0.77	0.07*
11	Protein disulfide-isomerase^●	P07237	4.76	4.00	57116	57000	1 : 0.98	0.03
12	Calreticulin^●	P27797	4.29	3.68	48142	53000	1 : 0.81	0.09*
13	Calumenin^●	O43852	4.47	3.70	37107	45000	1 : 0.92	0.11
14	Protein disulfide-isomerase A6^●	Q15084	4.95	4.00	48121	47000	1 : 0.94	0.08
15	Galectin-1^●	P09382	5.33	4.34	14716	14000	1 : 0.82	0.08*
16	Heat shock cognate 71 kDa protein	P11142	5.37	5.17	70898	65000	1 : 1.74	0.06*
17	Tubulin alpha-1A chain	Q71U36	4.94	5.17	50136	53000	1 : 1.92	0.04*
18	Actin cytoplasmic-1^●	P60709	5.29	5.17	41737	45000	1 : 1.86	0.11*
19	Protein S100-A6	P06703	5.32	4.40	10180	6000	1 : 0.78	0.03*
20	Neuron cytoplasmic protein 9.5	P09936	5.33	5.00	24824	26000	1 : 1.51	0.05*
21	Profilin-1^●	P07737	8.44	9.10	15054	15000	1 : 0.70	0.10*

^●Proteins with functions related to wound healing; SIR: spot intensity ratio HAF : HFF; *statistical significance between relative spot intensities (𝑛=2×3, 𝑃>0.005).