Role of ROBO4 Signalling in Developmental and Pathological Angiogenesis

Figure 2

SLIT2 mediated proangiogenic or antiangiogenic activity is dependent on recruitment of srGAP: interaction of SLIT2 with ROBO1 induces the recruitment of Rho GTPase activating proteins (srGAPs) to its CC3 domain which increases the intrinsic GTPase activity of CDC42 leading to reduced level of active CDC42. Reduction of active CDC42 level results in reduced actin polymerization and filopodia formation in endothelial cell. ROBO1–ROBO4 heterodimer formation hinders the binding of srGAP resulting in activation of CDC42, filopodia formation, and ultimate angiogenesis.