Cervical Cancer Cell Supernatants Induce a Phenotypic Switch from U937-Derived Macrophage-Activated M1 State into M2-Like Suppressor Phenotype with Change in Toll-Like Receptor Profile
Figure 5
Toll-like (TLR) -3, -7, and -9 expression in M1 macrophages treated with the supernatant of cervical cancer (CC) cells. U937-derived macrophages were activated with lipopolysaccharides (LPS) and treated or not with supernatant of HeLa, SiHa, and C-33A cells or Dexamethasone (DEX) for 7 days. After that, expression of CD163 and TLR-3, -7, and -9 was analyzed by flow cytometry (FC). Percentage of TLR-3, -7, and -9 expression in CD163– U937-derived macrophages activated with LPS (a). Geometric mean fluorescence intensity (MFI) values for TLR-3, -7, and -9 in CD163− U937-derived macrophages activated with LPS (b). Results are shown as % and MFI and represent the mean ± standard deviations (SD) of three independent experiments performed in triplicate. Statistical analysis, Student’s -test. Basal and LPS versus HeLa, SiHa, and C-33A groups. C33-A versus Basal and LPS groups. LPS versus Basal group.
