Cervical Cancer Cell Supernatants Induce a Phenotypic Switch from U937-Derived Macrophage-Activated M1 State into M2-Like Suppressor Phenotype with Change in Toll-Like Receptor Profile
Figure 6
The supernatant of cervical cancer (CC) cells induces the expression of Toll-like receptor (TLR)-3, -7, and -9 in M2-like macrophages (CD163+). U937-derived macrophages were activated with lipopolysaccharides (LPS) and treated or not with supernatant of HeLa, SiHa, and C-33A cells or Dexamethasone (DEX) for 7 days. After that, the expression of TLR-3, -7, and -9 was analyzed in CD163+ cells induced by the supernatant of CC cells by flow cytometry (FC). Percentage of TLR-3, -7, and -9 expression on M2-like macrophages (CD163+) (a). Geometric mean fluorescence intensity (MFI) for TLR-3, -7, and -9 in the different experimental groups (b). Results are shown as % and MFI and represent the mean ± standard deviations (SD) of three independent experiments performed in triplicate. Statistical analysis, Student’s -test. Basal and LPS groups versus HeLa, SiHa, and C-33A groups. Basal and LPS versus HeLa group. Basal and LPS versus HeLa and SiHa groups.
