Activation of J77A.1 Macrophages by Three Phospholipases A2 Isolated from Bothrops atrox Snake Venom
Effect of BaTX-I, BaTX-II, and BaPLA2 on J774A.1 macrophage lysosomal volume. 2 × 105 macrophages were incubated with the toxins (6 µg/mL) or RPMI (control) for 15, 30, and 60 minutes at 37°C in a humidified atmosphere of 5% CO2. After that, the macrophages were incubated with 0.04% neutral red. The incorporated dye was solubilized and the absorbance of the supernatant was determined at 505 nm in a spectrophotometer. Values represent the mean ± S.E.M. from 3 independent experiments. * compared with control (ANOVA).