Rho/ROCK Signal Cascade Mediates Asymmetric Dimethylarginine-Induced Vascular Smooth Muscle Cells Migration and Phenotype Change
Figure 5
ADMA induces morphological changes of VSMCs. Confluent VSMCs (starved for 48 h in FCS-free DMEM without L-arginine) were pretreated with Y27632 (or not) and then incubated with ADMA or without ADMA at the concentration of 10 μM for 12 h. F-Actin was stained with FITC-phalloidin and nucleolus was stained with PI.
