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BioMed Research International
Volume 2014, Article ID 684325, 10 pages
Research Article

Effects of Eicosapentaenoic Acid and Docosahexaenoic Acid on Chylomicron and VLDL Synthesis and Secretion in Caco-2 Cells

Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

Received 11 March 2014; Accepted 19 April 2014; Published 28 May 2014

Academic Editor: René Holm

Copyright © 2014 Yue Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The present research was undertaken to determine the effects of EPA (20 : 5 n-3) and DHA (22 : 6 n-3) on chylomicron and VLDL synthesis and secretion by Caco-2 cells. Cells were incubated for 12 to 36 h with 400 μM OA, EPA, and DHA; then 36 h was chosen for further study because EPA and DHA decreased de novo triglycerides synthesis in a longer incubation compared with OA  ( ). Neither the uptake nor oxidation was different in response to the respective fatty acids ( ). Compared with OA, intercellular and secreted nascent apolipoprotein B48 and B100 were decreased by EPA and DHA ( ). Both DHA and EPA resulted in a lower secretion of chylomicron and VLDL ( ). In contrast to OA, EPA and DHA were preferentially incorporated into phospholipids instead of triacylglycerols ( ). These discoveries demonstrated that exposure of DHA and EPA reduced the secretion of chylomicron and VLDL partly by regulating the synthesis of TG and apoB.