Effects of Eicosapentaenoic Acid and Docosahexaenoic Acid on Chylomicron and VLDL Synthesis and Secretion in Caco-2 Cells
Figure 1
Effect of OA, EPA, and DHA on Caco-2 cell triacyl[3H]glycerol accumulation and secretion. The cells plated on filter membranes and cultured for 21 days were incubated with DMEM containing delipidated FCS in the absence or presence of 400 μM OA, EPA, or EHA, respectively, for the time specified in the figure. The incorporation of [1,2,3-3H]glycerol into triacyl[3H]glycerol accumulated in cells (a) and secreted into basolateral medium (b) was estimated as described in Section 2. OA: oleic acid; EPA: eicosapentaenoic acid; DHA: docosahexaenoic acid. Data are expressed as mean ± SD of 6 different experiments. Means without a common letter differ between fatty acids, .
