Effects of Eicosapentaenoic Acid and Docosahexaenoic Acid on Chylomicron and VLDL Synthesis and Secretion in Caco-2 Cells

Figure 2

Percent uptake of EPA, DHA, and OA by Caco-2 cells. Caco-2 cells plated on filter membranes and cultured for 21 days were incubated in serum-free DME medium containing 400 μM [l-¹⁴C]OA, [l-¹⁴C]EPA, or [l-¹⁴C]DHA. After 12, 24, and 36 h of incubation, acid-precipitable products were determined from the apical medium (a), cells (b), and basolateral medium (c). The results are expressed as % of added acid-precipitable radioactivity/mg cell protein. Data are expressed as mean ± SD of 6 different experiments. Means without a common letter differ, 𝑃<0.01.

(a)

(b)

(c)