Effects of Eicosapentaenoic Acid and Docosahexaenoic Acid on Chylomicron and VLDL Synthesis and Secretion in Caco-2 Cells
Figure 5
Effect of OA, EPA, and DHA on Caco-2 cell nascent apolipoprotein synthesis and secretion. The cells plated on filter membranes and cultured for 21 days were incubated with 400 μM OA, EPA, or DHA for 35 h. After 35 hours, cells were incubated in methionine-free medium with albumin alone or respective fatty acid for 1 h. Labeled [S] methionine was added to the apical well. Apolipoproteins B-48 and B-100 were analyzed after the incubation with [S] methionine by SDS gel electrophoresis. The cells were harvested and basolateral medium was collected for de novo cell (a) and medium (b) apolipoprotein synthesis as described in experimental procedures. OA: oleic acid; EPA: eicosapentaenoic acid; DHA: docosahexaenoic acid. Data are expressed as mean ± SD of 6 different experiments. Means without a common letter differ between fatty acids, .
