Research Article

Mitogenic Effects of Phosphatidylcholine Nanoparticles on MCF-7 Breast Cancer Cells

Figure 1

Akt and mTOR phosphorylation and protein content. MCF-7 breast cancer cells were incubated for 24 hs with PC nanoparticles dispersed at 0.1 and 0.01% (w/v) in buffer pH 5.0 and buffer pH 7.0 or vehicle (Ct) in the absence ((a) and (c)) or presence ((b) and (d)) of serum. Representative results of immunoblots with anti-Akt and anti-phospho-Akt S473 ((a) and (b)) and anti-mTOR and anti-phospho-mTOR S2448 ((c) and (d)) are shown. Reprobing with anti-actin antibody demonstrated uniformity of protein loading in all lanes. Quantification of phosphorylated proteins was performed by scanning densitometry and expressed as percent of values measured for control, nonstimulated breast cancer cells (Ct). Data are expressed as the mean ± S.E.M. of the indicated number ( ) of different experiments. Statistical analysis was performed by ANOVA.