Mitogenic Effects of Phosphatidylcholine Nanoparticles on MCF-7 Breast Cancer Cells
Figure 6
EGFR immunocytochemistry. MCF-7 breast cancer cells were incubated for 24 hours with PC nanoparticles dispersed at 0.1 in buffer pH 7.0 or vehicle (Ct) in absence of serum. For EGFR immunocytochemistry, cells were washed, fixed, permeabilized, blocked, and incubated with the anti-EGFR antibody, the Cy3-conjugated secondary antibody, and Hoechst. Finally covers were mounted and examined by confocal microscopy. Images were obtained using sequential scanning. Representative images of EGFR, nuclear staining, merge, and bright field are shown.
